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Fig. 2

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ZDB-IMAGE-100427-14
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Figures for Iwase et al., 2007
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Figure Caption

Fig. 2 Specific Demethylation of Methylated H3K4 on Native Histone by SMCX, SMCY, Plu-1, and RBP2

(A and B) Calf thymus histones (3 μg) were incubated with 3 μg of purified, full-length SMCX, SMCY, RBP2, and PLU-1, respectively, and subjected to western blot analysis using antibodies that specifically recognize methylated histones. Heat-inactivated recombinant proteins were used as controls. Reduced signals were found only for H3K4me3 and H3K4me2 by SMCX and SMCY and for all three methylation states by Plu-1. Nonspecific signals were marked as asterisks.

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Reprinted from Cell, 128(6), Iwase, S., Lan, F., Bayliss, P., de la Torre-Ubieta, L., Huarte, M., Qi, H.H., Whetstine, J.R., Bonni, A., Roberts, T.M., and Shi, Y., The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases, 1077-1088, Copyright (2007) with permission from Elsevier. Full text @ Cell