ZFIN Image: Veldman et al., 2010, Fig. S3

Image

Figure Caption

Fig. S3 The Sp1 consensus sequence can compete for nuclear protein binding to the G/C-rich enhancer. Electrophoretic mobility shift assay showing the Sp1 consensus binding site probe can efficiently compete nuclear protein binding to the G/C-rich enhancer. Below the gel are shown the oligonucleotide sequences of consensus protein binding sites for myc-associated zinc finger protein (MAZ), GLI, specificity protein 1 (SP1), and smad interacting protein (SIP) used as unlabeled competitors. Consensus binding sites are underlined. The arrow marks specific protein binding. NS, Nonspecific binding; F, free probe.

Acknowledgments

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Reprinted from Molecular and cellular neurosciences, 43(4), Veldman, M.B., Bemben, M.A., and Goldman, D., Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish, 370-383, Copyright (2010) with permission from Elsevier. Full text @ Mol. Cell Neurosci.