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Fig. S6 IGF signaling is required for the progression of normally initiated regeneration. (A-I) Bright-field images of fins in a drug-shift experiment: 30 hours at normal conditions and 5 days with treatment. (A-C) Fins before inhibitor treatment at 30 hpa. (D-F) Fins after additional 5 days exposure to 5 μM AEW541 (E) and 5 μM ADW742 (F) reveal impaired regeneration in comparison with control treated with 0.05% DMSO (D). (G-I) Higher magnifications of the fin regenerates reveal aberrant differentiation of the skeletal rays and weaker pigmentation after inhibitor treatment (H,I) in comparison with control (G). (J) Quantification of the outgrowth size after the drug-shift experiment. n=3; *, P<0.001.