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Fig. 4 Early changes in differentiation following overexpression of Bmp2b. A: Expression of pax3 in a 12S non-heat-shocked control embryo. Pax3 is expressed by the anterior border cells (ABCs). B: Expression of Pax3 at 12S following heat-shock induction of Bmp2b overexpression at 3S. pax3 expression is no longer confined to the anterior cells, and is expressed throughout the somite. C: Expression of myoD in a 12S non-heat-shocked control embryo. Myod is expressed throughout the posterior somite, and excluded from the ABCs. D: Expression of myoD at 12S following heat-shock induction of Bmp2b overexpression at 3S. The domain of myoD expression is decreased. Red dots indicate somite boundaries. Scale bar = 50 μm.