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Fig. S8 Early non-muscle myosin II localization in mypt1 mutants. Fixed wild-type and mypt1 mutant embryos were stained with non-muscle myosin II antibody (green) and counterstained with propidium iodide to label nuclei (red). Representative images from four independent experiments. For each experiment, two to three embryos were imaged at each stage. (A,B) Whole-mount (A) and vibratome section (B) of a wild-type embryo at 19 hpf. (C,D) Whole-mount (C) and vibratome section (D) of mypt1 mutant at 19 hpf. (E) Diagram of embryo stage and region imaged in A-D. (F,G) Whole-mount (F) and vibratome section (G) of wild-type embryo at 21 hpf. (H,I) Whole-mount (H) and vibratome section (I) of mypt1 mutant embryo at 21 hpf. (J) Diagram of embryo stage and regions imaged in F-I. (A,C,F,H) Anterior is to the left. (B,D,E,G,I,J) Dorsal is to the top. Asterisks indicate ear. Scale bars: 50 μm.