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Fig. 3 Knockdown of smarcal1 causes developmental defects in zebrafish. (A–F) The gross morphology of 1 dpf and 2 dpf smarcal1 morphants. The morphology of uninjected and control MO injected embryos (8 ng per embryo) was largely normal. By 2 dpf, the embryos injected with smarcal1 MO (8 ng per embryo) showed growth defects including less dark pigment, unconsumed yolk sac and heart edema (F). (G–I) O-dianisidine staining of 2 dpf embryos showed reduced hemoglobin-positive cells on the yolk in smarcal1 morphants (I). Anterior is to the left.
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Reprinted from Developmental Biology, 339(1), Huang, C., Gu, S., Yu, P., Yu, F., Feng, C., Gao, N., and Du, J., Deficiency of smarcal1 causes cell cycle arrest and developmental abnormalities in zebrafish, 89-100, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.