|
Fig. S7 Maternal expression of kif5b RNA. (A,B) Maternal expression of kif5b. One-cell stage embryos (20 mpf) were fixed and hybridized with antisense (A) or sense control (B) probe. The kif5b probe was produced by RT-PCR. (C) RT-PCR. cDNA was generated from 1-cell stage embryos with (lane 2) or without (lane 1) reverse transcriptase, and a kif5b cDNA fragment was amplified by RT-PCR. Primers used for the kif5b detection are 5′-GATTCGGTAACCGGGGGAAGATGG-3′ and 5′-TCCACATTGACACACACAGTATTC-3′. (D,E) kif5b mRNA is ubiquitously distributed in a stage I oocyte as revealed by in situ hybridization of wild-type ovary sections with antisense (D) versus sense (E) probes. Scale bars: 200 μm in A,B; 50 μm in D,E.