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Fig. 3 Maternal expression of syntabulin rescues tkk mutants. (A) Structure of the rescue plasmid. Tol2, inverted repeats of Tol2 transposon; zpc, promoter of zone pellucida protein C; 3′ UTR, 3′ untranslated region; pAS, polyadenylation signal. (B) Rescue by syntabulin cDNA transgene. The numbers of transgenic (green) and non-transgenic (orange) zebrafish embryos showing each phenotype are tabulated, and the percentages are shown in the bar chart. (C,D) Expression of dha in tkk (D; 8%, n=25) and transgene-rescued (C, GFP+; 48%, n=33) embryos at the late blastula stage. (E,F) Localization of syntabulin at the 1-cell stage in a transgene-rescued (E; 67%, n=27) versus control (F; 100%, n=23) embryo. Scale bars: 200 μm.