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Fig. S7 xav mutants display electrophysiological properties in the muscle, comparable to WT. A–C. Miniature excitatory postsynaptic current (mEPC) recordings from fast muscles showed a significant reduction in the mEPC rise time (WT 0.122±0.007 ms, xav 0.096±0.002 ms, N = 6, Student′s t test, * p<0.05) and the frequency (WT 0.36±0.07 Hz, xav 0.10±0.05 Hz, N = 6; Student′s t test, * p<0.05), but no statistical difference in terms of amplitude and exponential decay time. N = 6–8 embryos, 2 carrier pairs. D. Muscle fibers from xav embryos fire action potentials after exogenous stimulation, comparable in amplitude and shape to action potentials recorded from muscle fibers from WT embryos. There is no significant difference in terms of action potential amplitude, threshold or half width. N = 5 embryos, 2 carrier pairs.