Fig. 5 Overexpression of unplugged/MuSK in WT embryos does not perturb synaptogenesis.
21-somite stage (A–F) or 27 hpf (G–L) embryos after HS treatment. (A–F) Wildtype (WT) embryos, Tg(hsp70l:unplugged SV-1myc) embryos, and Tg(hsp70:unplugged SV1-myc); unpluggedbr307/br307 embryos received HS from the 10- to 21-somite stage The AChR prepatterning zone appears slightly expanded in (D) compared to (B and F). White dashed lines mark the boundaries of each segment. (G–L) WT embryos, hsp70l:unplugged SV-myc embryos, (I and J) or hsp70l:unplugged FL-myc embryos (K and L) were heat shocked from the 10-somite stage until 27 hpf. Motor axons and neuromuscular synapses appear normal in the embryos expressing the transgene (I–L). Scale bars: 20 μM.