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Figure Caption
Fig. 5 Mutating the motif TTTCAG impairs the forebrain enhancer activity of CNE2.01.2. (A) Alignment of zebrafish and human CNE 2.01.2. Abbreviations: D, distal to the transcription start site; P, proximal to the transcription start site. (B) Functional validation of de novo predicted motifs in the CNE2.01.2. Reporter activity of (1) The CNE containing the wildtype copies of the motif TTTCAG, or mutations in the following motif: (2) either the distal or the proximal copy of the motif; (3) Mutating both copies of the motif.
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Reprinted from Developmental Biology, 337(2), Li, Q., Ritter, D., Yang, N., Dong, Z., Li, H., Chuang, J.H., and Guo, S., A systematic approach to identify functional motifs within vertebrate developmental enhancers, 484-495, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.