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Fig. 5 Gut organogenesis fails in the mga morphant embryos. Shown are representative control or morphant (MO1) embryos derived from ef1a:gfp transgenic fish used to image endoderm/gut morphogenesis. A, B: The comparison at 48 hpf shows relatively normal budding of the pancreas and liver suggesting that early organ budding is normal. C, D: By 3 dpf, the mga morphants show a significant decrease in the growth of liver and intestine tissue. E, F: By 5 dfp, the mga morphant embryos completely lack GFP-labeled endoderm-derived organs including the pancreas (dashed line in E), liver (dashed line in F), intestine, and swim bladder. Views are: (A, B) dorsal, anterior to the left, (C, D) lateral, dorsal to the top and anterior to the left, (E) lateral, dorsal to the top and anterior to the right, (F) lateral, dorsal to the top and anterior to the left. L, liver; P, pancreas; IB, intestinal bulb; SB, swim bladder.