ZFIN Image: Kusik et al., 2010, Fig. 2

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Figure Caption

Fig. 2 The 708-bp promoter is sufficient to promote expression in developing but not regenerating retina. Native GFP expression (green) and DAPI staining (blue) in transverse sections of embryonic, larval, and adult retinas. The GfG43SA and GfG43-708 constructs both express GFP in the retinal ganglion cell layer (gcl) and their axons (*) observed in the fiber layer at 48 hpf (A,E) and in the inner plexiform layer (ipl) within the retina at 96 hpf (B, F). In control and regenerating adult retina (7 days post-crush) of the GfG43SA line, low levels of GFP are observed in control retina (C) and high levels in the GCL and fiber layer of regenerating retina (D). In the GfG43-708 line, there is no GFP expression in the GCL of either control (G) or regenerating (H) retina. Developing retina scale bar (F) = 25 μm. Adult retina scale bar (H) = 50 μm.

Acknowledgments

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