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Figure Caption
Fig. S1 sip1a splice morpholino is effective in eliminating wild-type sip1a mRNA. Reverse transcriptase-polymerase chain reaction (RT-PCR) of mRNA from staged sip1a splice MO injected embryos with sip1a specific primers reveals that the smaller misspliced product is detected by dome stage (4.3 hours postfertilization [hpf]). By 75% epiboly (8 hpf), no wild-type mRNA is detected.
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