ZFIN Image: Codina et al., 2010, Fig. 4

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Figure Caption

Fig. 4 Effects of smyhc1 knockdown or hsp90α1 mutation on myosin thick filament organization in skeletal muscles of zebrafish embryos.

A–C. Anti-MyHC antibody (F59) staining shows the organization of thick filaments in trunk slow muscles of control (A), smyhc1 knockdown (B), or slo^tu44c mutant (C) embryos at 48 hpf. D–F. Anti-MLC antibody (F310) staining shows the organization of thick filaments in trunk fast muscles of control (D), smyhc1 knockdown (E), or slo^tu44c mutant (F) embryos at 72 hpf. Note, fast fibers project with a 30 degree angle with respect to the axial structure, whereas slow fibers project in parallel to the axial structure. G, H. Anti-MyHC antibody (F59) staining shows the rescue of thick filaments in smyhc1 knockdown zebrafish embryos co-injected with ef1a:smyhc1 DNA construct (G), or ATG-MO alone (H). Scale bar = 25 μm in A, 10 μm in G.

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Acknowledgments

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