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Figure Caption
Fig. 4 piRNA Expression
(A) Northern blot for piRNA piR-31 at various developmental stages is shown.
(B) shows piR-31 and piR-80 in situ hybridization on ovary. Open arrows indicate stage I oocytes, displaying staining in the cytoplasm. Closed arrows indicate granules along the cortex of stage III/IV oocytes. Scale bars are 50 μm.
(C) piR-80 in situ hybridization on testis is shown. Cytoplasmic staining is observed in all stages, including premeiotic stages, except for sperm.
(D) GypsyDR1 in situ hybridization on ovary is shown. Nuclear staining is observed in stage I oocytes and rapidly fades in older stages.
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Reprinted from Cell, 129(1), Houwing, S., Kamminga, L.M., Berezikov, E., Cronembold, D., Girard, A., van den Elst, H., Filippov, D.V., Blaser, H., Raz, E., Moens, C.B., Plasterk, R.H., Hannon, G.J., Draper, B.W., and Ketting, R.F., A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish, 69-82, Copyright (2007) with permission from Elsevier. Full text @ Cell