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Fig. 7 Effect of PAR tethering peptides on thrombocyte function.
Top panel: Effect of PAR tethering peptides on thrombocyte aggregation. Plate tilt assay measuring thrombocyte function. (A) DAEFRH (control peptide). (B) TDEQTE (PAR1-21D). (C) SFSGFF (PAR1-5A). (D) PTQQTL (PAR1-21A). (E) SLVVIQ (PAR2-21A). (F) GFTGEE (PAR2-21D). Note the aggregated blood in (C), (D) and (E) is firmer than the control blood (A) and blood (B) and (F). 5 ng of each peptide was used. Middle panel: Measurement of functional activity of PAR tethering peptides on thrombocytes by annexin V binding. 5 ng of each tethering peptide was used. The intensity of images from FITC-annexin fluorescence (green) was measured as mean pixels using Adobe Photoshop software version 7.0 (T test, n = 12). Bottom panel: Effect of SLVVIQ tethering peptides on gill bleeding. The zebrafish were subjected to the gill bleeding assay as described in Materials and Methods with the following conditions: (A) 50 ng of DAEFRH (control peptide) and (B) 50 ng of SLVVIQ (PAR2-21A).