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Fig. S1 Hedgehog-responsive genes are expressed in ventral jaw cartilages. (A) Ptc2 expression at 4 dpf. Strong expression can be seen in the ceratohyal and Meckel′s cartilage (black arrows), and in the branchial arches in the chondrocyte precursors (blue arrows). (B) Expression of the Hh transcription factors gli1, gli2 and gli3 in sibling and ptc2-/- fish at 4 dpf. Both gli1 and gli3 appear upregulated in the mutants, compared with siblings, particularly in the cartilage precursors of the Meckel′s cartilage (red arrows) and ceratohyal (black arrow in gli3 pictures). By contrast, gli2 expression levels appear to be unchanged or slightly weaker in the mutants than in the siblings.