ZFIN Image: Joshi et al., 2009, Fig. S6

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Figure Caption

Fig. S6 APP₆₉₅ and APP-CΔ18 are present at the plasma membrane in HEK 293 cells. Confocal images of HEK 293 cells transiently transfected with pCS2 + APP₆₉₅ or the deletion mutant pCS2 + APP-CΔ18. The cells were fixed in paraformaldehyde and probed with 6E10 antibody against the Aβ region or with 22C11 antibody against the extracellular region of APP, followed by an anti-mouse secondary conjugated with Alexa 594. The immunofluorescence images show that full-length APP₆₉₅ (A) or CΔ18 truncation protein (B) can be detected at the plasma membrane when detected with 6E10 antibody. The immunofluorescence pattern is indistinguishable between APP₆₉₅ and CD18 (white arrowheads). 22C11 antibody that recognizes the N-terminal domain of APP gave a similar staining pattern (C, D).

Acknowledgments

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Reprinted from Developmental Biology, 335(1), Joshi, P., Liang, J.O., Dimonte, K., Sullivan, J., and Pimplikar, S.W., Amyloid precursor protein is required for convergent-extension movements during Zebrafish development, 1-11, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.