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Fig. S4 pax2a patterning defects are detected prior to fgf patterning defects in zic2a morphants. fgf8a expression is normal at 16s in control (A, 11/11 embryos) and zic2a morphants (B, 8/8 embryos). Similarly, spry4 is unaffected at 16s in control (C, 21/21 embryos, n=2) or zic2a morphants (D, 22/24 embryos, n=2). pax2a is expressed broadly in the OS and retinal primoridia at 16s (E, 15/15 embryos, n=2), and might be slightly expanded in zic2a morphants (F, 9/21 embryos, n=2). At 19s, fgf8a is weakly expressed in the presumptive OS (G, 12/12 embryos), and its expression is unaffected by knocking-down Zic2a (H, 9/9 embryos). spry4 is expressed in a broader domain than fgf8a (I, 20/20 embryos, n=2), and also appears unaffected in zic2a morphants (J, 21/25 embryos, n=2). pax2a expression has begun retracting from the retinal primordium by 19s in control embryos (K, 8/18, n=2), but not in zic2aMO-injected embryos (L, 9/15 embryos, n=2). Arrowheads mark the OS. All embryos are shown in ventral view, anterior to the left. Embryos in A-F are at 16s, while embryos in G-L are at 19s.