ZFIN Image: Green et al., 2009, Fig. S1

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Figure Caption

Fig. S1 ISH for markers of cartilage (col2a1) and bone (col10a1, osx) was performed on 120 hpf heterozygous rapunzel embryos and age-matched wild type siblings. Genotype was confirmed by sequencing following ISH (not shown). No differences in expression between rapunzel mutants and their wild type siblings were observed for any of the 3 genes. A, B. col2a1 expression is seen in the pharyngeal arches (pa), otic vesicle (ov), endochondral disc (ed) and fin fold (arrow). Expression of col10a1 (C, D) and osx (E, F) is localized to the cleithrum (cl), opercle (op), parasphenoid (ps), and brachiostegal ray (bs). Similar results were seen at 48, 72, and 96 hpf (not shown).

Acknowledgments

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Reprinted from Developmental Biology, 334(1), Green, J., Taylor, J.J., Hindes, A., Johnson, S.L., and Goldsmith, M.I., A gain of function mutation causing skeletal overgrowth in the rapunzel mutant, 224-234, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.