Fig. 4

Fig. 4 fezf2-GFP+ cells colocalize with markers of neurons and proliferation in the olfactory bulb. (A) Coronal section through the olfactory bulb showing double-labeling of fezf2-GFP (green) and BLBP (red) (20x magnification). (A′–A″′) Some fezf2-GFP+ cells colocalize with neural stem cell/astrocyte marker BLBP (arrowhead), suggesting that these are either local neural stem cells or differentiated astrocytes within the olfactory bulb (40x magnification). (A″′) Inset shows colocalization in a single cell at higher magnification. (B) Coronal section showing fezf2-GFP (green) and GFAP (red) labeling in a single olfactory bulb. (B′–B″′) Some fezf2-GFP+ cells colocalize with GFAP (arrowhead), suggesting that these are either local neural stem cells or differentiated astrocytes (40x magnification). (B″′) Inset shows colocalization in a single cell at higher magnification. (C) Coronal section showing double-labeling of fezf2-GFP (green) and PCNA (red) (20x magnification). (C′–C″′) Some fezf2-GFP+ cells colocalize with proliferation marker PCNA (arrowhead) (40x magnification). (C″′) Inset shows colocalization in a single cell at higher magnification. (D) Coronal section showing double-labeling of fezf2-GFP (green) and BrdU (red) in the caudal olfactory bulb (20x magnification). (D′–D″′) Some medio-dorsal fezf2-GFP+ cells colocalize with BrdU (S-phase marker) (arrowhead) (40x magnification). (D″′) Inset shows colocalization at higher magnification. (E) Coronal section showing double-labeling of fezf2-GFP (green) and Hu (red) (20x magnification). (E′–E″′) Closer view of the boxed region shows that some fezf2-GFP+ cells colocalize with neuronal marker Hu (arrowhead) (40x magnification). (E″′) Inset shows colocalization in a single cell (depicted by arrowhead) at higher magnification.