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Fig. S2 Transcript and protein levels are equal in other brain regions (A-D) and in hypothalamic catecholamine neurons (E-F) in WT and nsf mutant 5dpf larvae. (A-D) Whole-mount expression patterns are shown for pomc (A-B) and emx (C-D) in WT and nsfst53 zebrafish (n≥8 per marker per genotype). Maintained expression of pomc in nsf mutant pituitary is noted (black arrowhead). (E-F) Tyrosine hydroxylase protein levels in hypothalamic neurons is shown in sectioned WT and nsfst53 zebrafish (n=7 ea). Cell bodies are shown with DAPI stain.
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