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Fig. S4 adss and gmps morpholino efficacy. (A) RT-PCR for adss using two primer sets that flank the intron 1/exon 2 targeted region of the transcript. Both primer sets do not amplify any transcript from adss-MO-injected embryos at 24 hpf. wnt5 primers are used as a positive control for each cDNA. (B) RT-PCR for gmps using a primer set flanking the exon 2/intron 2 targeted region of the transcript. gmps-MO injection leads to a splicing defect in gmps transcripts at 24 hpf. (C) gmps-MO injection leads to the removal of an 81 bp portion of exon 2 (exon 2 sequence in black, excised portion in red).
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