Fig. 7

Fig. 7 Pigment-containing organelles and cell types are normal in gart and paics mutants. (A-I) High magnification images of transverse histological sections through wild-type (A,B), gart (D,E) and paics (G,H) eyes indicating the presence of melanosomes (black arrow), pterinosomes (yellow arrow) and iridosomes (red arrow) in the pigmented iris (A,D,G) and of melanosomes and iridosomes in the RPE and sclera (B,E,H). (C,F,I) TEM images of the iris of wild type (C), gart (F) and paics (I). (J-O) In situ hybridization for pigment cell types in representative embryos derived from gart (J,L,N) and paics (K,M,O) heterozygous incrosses. (J,K) dct marks melanocytes and melanosomes in the RPE. (L,M) gch marks xanthophores. (N,O) PNPase marks iridophores. Inset in N and O, high magnification view of iridophores in the eye (arrows). No differences in the number or distribution of pigment cells were observed between wild-type and mutant embryos (not shown).