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Figure Caption
Fig. S5 npy transcripts are absent but NPY protein is accumulated in nsf mutant larvae. Whole-mount staining for npy expression and immunocytochemistry for NPY protein in wild type and nsfst53 5dpf zebrafish is shown (n=5 per experiment per genotype). Loss of transcript (black arrowhead) is noted. The contrast between the decreased NPY levels in peripheral projections (white arrowhead) but accumulation of NPY in the soma of nsf mutant zebrafish and the levels observed in WT is highlighted.
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