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Fig. S4 Cell death is observed in sensory neurons in nsf mutant larvae. DAPI stain in the hypothalamus (A, B) and the posterior tuberculum of wild type and nsfst53 sectioned (12 μm horizontal sections) 5dpf zebrafish is shown. Morphological changes associated with cell death, such as cell asymmetry and chromatin condensation is noted (white arrowheads; D). Cell death is not observed in the hypothalamus (A,B), but is observed in olfactory terminals (F), otic capsule (H) and photoreceptor cells (J) of mutant zebrafish. Images representative of duplicate experiments, n≥5 per genotype.
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