ZFIN Image: Martin et al., 2009, Fig. 2

Image

Figure Caption

Fig. 2 Proliferative cells are seen in the atrioventricular (AV) boundary at 6 and 16 days postfertilization (dpf), but not between these times. A-D: Five micrometer paraffin sections of flk1::GFP transgene-carrying zebrafish embryos/larvae were imaged to identify proliferating cells in the AV boundary and intra-luminal structure. Atria (A) and ventricles (V) are labeled (yellow letters), and yellow lines outline the extent of the AV structure (cushion and/or valve). Staining identifies cell nuclei (DAPI; blue), proliferative cells (anti-bromodeoxyuridine [BrdU]; red), and endothelial cells (anti-GFP; green). (DAPI not shown in panel D to emphasize BrdU-positive cells). Proliferative cells are seen in the developing valves at 6 dpf (A) and 16 dpf (D), but not 8 and 12 dpf (B,C). Scale bar = 20 μm.

Acknowledgments

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