Quantitative analyses. Representative semiquantitative RT-PCR results for the gfp (A), heart-specific markers vmhc and cmlc2 (B) in MO-injected embryos at 48 hpf. The assay used 1 μg of total RNA for the RT reaction. Control experiment was performed with 1 μg (1X) and 2 μg (2X) of total RNA (C). We consistently found significant decrease in expression levels of vmhc and gfp when comparing Mock and GATA4 and -6 double-deficient embryos (MO4+6). (D) The expression level of endogenous vmhc and cmlc2 mRNA after 48 h of injection of antisense morpholino nucleotides (MOs) against GATA4 and GATA6. Intensities of bands from three independent semi-quantitative RT-PCR experiments were measured (P < 0.05)
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