ZFIN Image: Lin et al., 1994, Fig. 2

Image

Figure Caption

Fig. 2 Expression of lacZ in F1 transgenic embryos. F1 embryos from line EF-head were fixed at the early gastrula and stained with X-Gal. Three embryos which express lacZ show readily discernible blue staining (A). Expression patterns of lines EF-blue (B), EF-head (C), EF-patchy (D), and EF-neuron (E) are shown at different developmental stages. Expression of lacZ in embryos from line EF-neuron is restricted to the CaP motoneurons in the spinal cord (F, G, and H). sc, spinal cord. n, notochord. The distribution of lacZ-expressing CaP cells is variable between embryos as shown from the dorsal view (I).

Acknowledgments

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Reprinted from Developmental Biology, 161, Lin, S., Yang, S., and Hopkins, N., lacZ expression in germline transgenic zebrafish can be detected in living embryos, 77-83, Copyright (1994) with permission from Elsevier. Full text @ Dev. Biol.