ZFIN Image: Kitaguchi et al., 2009, Fig. S1

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Figure Caption

Fig. S1 (A) Synthetic mRNA (250 pg) for C/ebp1-Nterminal-EGFP containing c/ebp1 MOs target sequences was injected into one blastomere of one- or two-cell stage embryos. Subsequently, morpholinos (c/ebp1 MO, 10 ng; c/ebp1 5mMO, 10 ng; c/ebp1 MO2, 2 ng) were injected into the yolk. The c/ebp1 5mMO is a 5 base mis-matched control morpholino for c/ebp1 MO. The expression of C/ebp1-Nterminal-EGFP at shield stage was effectively suppressed in c/ebp1 MO- and c/ebp1 MO2-injected embryos, but not in c/ebp1 5mMO-injected embryo. (B) lyz expression was inhibited in c/ebp1 MO- and c/ebp1 MO2-injected embryos, while c/ebp1 5mMO injection did not affect lyz expression.

Acknowledgments

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Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.