ZFIN Image: Vasil et al., 2009, Fig. 12

Image

Figure Caption

Fig. 12 Endothelial cell death does not play a predominant role in zebrafish circulatory collapse and recovery following a 1 ng dose of PlcHR.

(Tg(Fli:EGFP) [41]) and (Tg(Fli:nucEGFP) [Roman et al., 2002]) embryos were injected with 1 or 2 ng of PlcHR, or 2 ng of its catalytic mutant as before (A?C). (A?C) Acridine orange staining detected minimal cell death following the injection of 1 ng PlcHR (A′,B′), ISVs collapsed, but did not regress as in the 2 ng dose (C′), and the endothelial nuclei did not decrease [(A″,B″) and data not shown]. (C,C′,C″) The 2 ng PlcHR dose generated increased cell death, vessel regression, and reduced endothelial cell nuclei. Scale bar indicates 100 μm.

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