ZFIN Image: Castets et al., 2009, Fig. S3

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Figure Caption

Fig. S3 Effect of netrin-1a and UNC5B Morpholinos In Zebrafish Embryos
(A) Netrin-1b does not seem to be involved in developmental angiogenesis in zebrafish. Translation-blocking netrin-1b morpholino has no significant defect on developmental angiogenesis. Percentage of embryos without defects and total number of embryos analysed are indicated. Phylogenic study of netrin-1 otholog genes. ML on protein sequences, under JTT model, with 100 bootstrap replicates. Netrin-1 orthologs in zebrafish, netrin-1a and netrin-1b, are underlined.
(B) RT-PCR analysis of netrin-1a and UNC5B expression in zebrafish embryos.
(C) Third netrin-1a blocking morpholinos lead to similar vascular defects than both described in Figure 3. Percentage of embryos with ISVs defects and total number of embryos analysed are indicated.
(D) Netrin-1a silencing leads to increase caspase-3 activity in whole zebrafish embryos. Caspase-3 activity is expressed as a relative index referred to embryos injected with control MO. Means and s.e.m. are calculated from three independent experiments.

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Acknowledgments

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Reprinted from Developmental Cell, 16(4), Castets, M., Coissieux, M.M., Delloye-Bourgeois, C., Bernard, L., Delcros, J.G., Bernet, A., Laudet, V., and Mehlen, P., Inhibition of Endothelial Cell Apoptosis by Netrin-1 during Angiogenesis, 614-620, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell