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Fig. S10 fgf8 MO injection results in reduced Fgf signaling. (A,A′,B,B′) Live images of control embryos (A,A′) or fgf8 MO-injected embryos (B,B′). As previously shown by Draper et al. (Draper et al., 2001), the fgf8 MO used generates a range of phenotypic classes (Draper et al., 2001). In our experiments, injected embryos exhibited phenotypes similar to those described as class 1-3 phenotypes, most typically the class 2 phenotype (Draper et al., 2001). fgf8 MO-injected embryos were selected for the absence of the midbrain-hindbrain boundary, indicated by the black arrowhead. Approximately 40% of the injected embryos presented this phenotype and only these were imaged in the developmental timing assay.