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Fig. 3 Immunohistochemical identification of Wnt-responsive cells in the 50 hpf embryonic hypothalamus. (A) Lateral view of TOP:dGFP whole-mount brain stained with GFP antibody, observed in a 100-μm confocal projection. (B, C) Triple labeling for TOP:dGFP, HuC/D or PCNA, and Topro3, observed in a single confocal slice. (D–I) Twelve-micron cryosections immunostained for the markers listed in each panel. Positions of cross sections are indicated in panel (A). Boxed region is shown at higher magnification in lower right corner, and circled cells are double labeled. GFP staining partially overlaps with HuC/D, PCNA, and 5-HT, but not with TH, GABA, or GFAP. T, telencephalon; TeO, tectum opticum; DT, dorsal thalamus (thalamus); Hy, hypothalamus. Scale bars: (A, B) 100 μm; (D) 50μm.