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Fig. 1 Genistein induces apoptosis during zebrafish development (A) 24 hpf photo of an untreated embryo and an embryo treated with 10 μM of genistein showing necrosis in the posterior hindbrain and in the anterior spinal cord marked by the white bracket (B).
(C–H) Apoptotic cell detection at 24 hpf using acridine orange. (C) Control embryo with few apoptotic cells in the midbrain (arrowhead) and the hindbrain (arrow). (D, E) Embryo exposed to 2.5 and 10 μM of genistein showing an increased number of stained cells especially in the hindbrain and a high number of apoptotic cells in the hindbrain and anterior spinal cord when high concentrations of genistein are used (bracket in F). (F–H) Apoptosis mainly found in the hindbrain (arrows) induced by compounds related to genistein: genistin (F), apigenin (G) and daidzein (H) each at 10 μM. Using daidzein, numerous apoptotic cells are detected in the forebrain in comparison to what is observed with the other compounds.