Fig. 7

Fig. 7 The PGE2/wnt Interaction Is a Master Regulator of Liver Regeneration

(A–D) Representative photomicrographs of en bloc dissections following liver resections at day 3 are shown; the liver is highlighted by a yellow dotted line, the resection site by a blue line, and the black arrow indicates the amount of liver regrowth. Wnt activation in APC mutant zebrafish enhanced liver regeneration compared to WT. Indo stymied liver regeneration.

(E) Quantification of zebrafish liver regeneration showed significant differences across treatment groups; ^*sig versus con, ^**sig versus APC^+/-, ANOVA, p < 0.001, n ≥ 6.

(F) qPCR for cyclin D1 gene expression; effects are coordinately regulated by the PGE2/wnt interaction; ^*sig versus con, ^**sig versus APC, ANOVA, p < 0.001, n = 7.

(G–J) wnt and PGE2 modulation has significant effects on murine liver regeneration following 2/3 partial hepatectomy. APC^Min mice exhibit enhanced β-catenin staining (left panel), particularly in the periportal areas (top middle panel), with noticeable nuclear staining (bottom middle panel). BrdU incorporation (top right panel) and cyclin D1 staining (bottom right panel) indicated enhanced regenerative activity. Indo diminished global β-catenin staining (left and top middle panels), excluded β-catenin from the nuclei (bottom middle panels), and resulted in a corresponding decrease of both BrdU incorporation and cyclin D1.

(K and L) Quantification of BrdU incorporation and cyclinD1 staining in corresponding serial sections of regenerating livers; ^*sig versus con, ^**sig versus APC, ANOVA, p < 0.05, n = 10 sections/treatment.

Data represented as mean ± SD.