Fig. 5

Fig. 5 Znr2/Sqt can signal to the blastoderm from the YSL. Embryos in A–D, G, and H were coinjected with 100–200 pg znr2 RNA and fluorescent rhodamine dextran (red) in the YSL at the high to oblong stages. (A) Shield stage. Arrowhead indicates enlarged shield. (B) Fluorescence image showing exclusion of injected material from the embryo. (C) Nomarski image of a four-somite-stage embryo that appeared similar to A at shield stage. Note the altered embryonic morphology compared to the uninjected control in E. (D) Fluorescence image showing exclusion of injected material from the embryo. (E) Nomarski image of a six-somite-stage uninjected control embryo. (F–H) gsc expression at shield stage; arrowheads mark expression boundaries. Animal views. (F) Uninjected control. (G) znr2 RNA (100 pg), broadened gsc expression. (H) znr2 RNA (200 pg), gsc is expressed in two very broad domains. Note that examination under fluorescence of embryos in G and H showed no rhodamine dextran-positive cells in the gsc-expressing embryonic blastoderm.