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Fig. 4 Znr2/Sqt induces secondary axes. (A) Uninjected wild-type sibling (bud stage; lateral view, anterior left) showing hatching gland (arrow) and notochord (arrowhead). (B) znr2 RNA (10 pg) injected into the yolk at the 2- to 4-cell stage. Note duplicated hatching gland (hgg1-positive, red; indicated by arrows) and notochord (ntl-positive, purple, indicated by arrowheads; anterior left). (C) znr2 RNA (2.5 pg) injected into one marginal cell at the 16-cell stage processed for ntl expression; the duplicated notochord is indicated (arrowheads; posterior view). (D) znr2 RNA (5 pg) injected into one marginal cell at the 16-cell stage; both axes contain forebrain (arrows) and neuronal precursors along the length of the body as shown by in situ analysis for zHuC (anterior left).
Reprinted from Developmental Biology, 204, Erter, C.E., Solnica-Krezel, L., and Wright, C.V.E., Zebrafish nodal-related 2 encodes an early mesendodermal inducer signaling from the extraembryonic yolk syncytial layer, 361-372, Copyright (1998) with permission from Elsevier. Full text @ Dev. Biol.