IMAGE

Fig. S5

ID
ZDB-IMAGE-090320-78
Publication
Jing et al., 2009 - Wnt signals organize synaptic prepattern and axon guidance through the zebrafish unplugged/MuSK receptor
All Figures
Figures for Jing et al., 2009
Image
Figure Caption

Fig. S5 Unplugged interacts with zebrafish Dishevelled.
(A) The intracellular domain of unplugged /MuSK (Unpic) was cloned into a bait plasmid pGBKT7. The PDZ and DEP domains of zebrafish dishevelled 2 and dishevelled 3 were cloned into a prey plasmid pGADT7. Two-hybrid interactions were preformed using SD/-Ade/-His/-Leu/-Trp media to screen for reporter genes: ADE2 and HIS3 (+++: fast growth. ++: intermediate growth. -: no growth). (B, C): The morphology and differentiation of adaxial cells expressing Myc-Xdsh-DEP+ appears normal. 28 hpf embryo injected with symhc1:myc-Xdsh-DEP+ and stained with mAb anti-Myc (red) and F59 (green, adaxial cells).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron
Your Input Welcome
We welcome your input and comments. Please use this form to report issues or recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.