Fig. 4

Fig. 4 Lateral views of HCNE-test transgenic zebrafish embryos at 2 days post fertilization. Transgenes are labeled by locus name (pax6a/b/PAX6), organism (zebrafish = dr/human = hs) and HCNE number. (A, B) Show transgenes made with zebrafish HCNE 1 expressing GFP in optic tectum, pectoral fins and hindbrain vs. human element driving expression in dorsal root ganglia and hindbrain, which indicate its functional connection to WT1 expressed in these domains. HCNE 2 in (C, D) directs expression mainly to hindbrain with both zebrafish and human elements. Embryos in (E) made with human sequence PAX6_hs6 express in optic tectum, tegmentum, hindbrain, spinal chord and retina, embryo (F) expressing in dorsal diencephalon (PAX6_hs7). Sub-sequence pax6a_dr6A (as seen in Fig. 6B) drives expression only in optic tectum and retina but the human homologous PAX_hs6A element directs expression to optic tectum, hindbrain, spinal chord, lateral line and lens.