Fig. 5

Fig. 5 Anteroposterior patterning of the brain is not affected by ethanol treatment. (A to C) pax-6 mRNA expression in untreated control (A) and ethanol-treated embryos (B, C). pax-6 expressions in the dorsal diencephalon (d) and hindbrain are indicated by arrowheads. (D to F) pax-2 expression in control (D) and ethanol-treated embryos (E, F). pax-2 expression in the optic stalks and ventral eye (arrowhead with dot) is progressively lost with increasing severity of cyclopia. In contrast, pax-2 expression in the posterior midbrain (arrowhead) and in the hindbrain is unaffected even in severely affected embryos. Orientation of embryos is anterior to the left; view onto dorsal (A, B) and lateral views (C to F).