ZFIN Image: Hans et al., 2009, Fig. 4

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Figure Caption

Fig. 4 Dose-dependent recombination in the Tg(pax2a:CreER^T2)^#19 and Tg(pax2a:CreER^T2)^#45 lines by TAM.

(a) Double transgenic embryos bearing the red-to-green reporter and the Tg(pax2a:CreER^T2)^#19 alleles show strong EGFP expression in the diencephalon after application of 5 μM TAM at mid-gastrulation stages. Application of 0.5 μM TAM or 0.05 μM TAM at the same stage, results in reduced EGFP expression or single EGFP-positive cells, respectively. (b) Double transgenic embryos bearing the red-to-green reporter and the Tg(pax2a:CreER^T2)^#45 alleles show strong EGFP expression in rhombomere 3 and 5 after application of 5 μM TAM at mid-gastrulation stages. Application of 0.5 μM TAM at the same stage, results in single EGFP-positive cells. a, b Dorsal views of double transgenic embryos at 24 hpf. Scale bar, 30 μm.

Acknowledgments

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