ZFIN Image: Hans et al., 2009, Fig. 3

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Figure Caption

Fig. 3 Kinetics of ligand-dependent Cre-mediated recombination.

(a) Expression of CreER^T2 in the Tg(pax2a:CreER^T2)^#19 line at the 12-somite stage revealed by in situ hybridization. (b) Control embryos treated with DMSO never show any EGFP. (c) Immunofluorescence staining with antibodies to EGFP is detectable 4 hours after application of TAM and expanded further after 6 hours. (d) Onset of EGFP expression by immunofluorescence staining is detected after 2 hours and expanded further after 4 and 6 hours after application of 4-OHT. a–d Dorsal views of double transgenic embryos at 12-, 16-, 20 and 24-somite stage (15, 17, 19 and 21 hpf). Abbreviations: f, forebrain; e, eye anlage; h, hours; m, midbrain. Scale bar, 30 μm.

Acknowledgments

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