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Figure Caption
Fig. 1 CB1 mRNA localization detected by Whole-mount in situ hybridization in whole zebrafish ovary. (A) Whole ovary transmitted light image; (A′) whole ovary green fluorescence image showing the localization of CB1 antisense mRNA probe; (A″) overlap of both previous images: stages III and IV showed the most abundant concentration of CB1 mRNA. The circle indicates the mature eggs with no fluorescence signal. (B) Negative control was performed with antisense probe pre-adsorbed with the sense probe in excess.
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Reprinted from Molecular and Cellular Endocrinology, 299(2), Migliarini, B., and Carnevali, O., A novel role for the endocannabinoid system during zebrafish development, 172-177, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.