Fig. 3 Abnormal histopathology in 72 hpf myotubularin morphants.
(A) H/E stained longitudinal myofibers from myotubular myopathy (MTM) and age matched control (CTL) human muscle biopsies. Arrows point to abnormal nuclei. (B) H/E stained longitudinal myofibers from control (CTL MO) and myotubularin (MTM MO) morphant 72 hpf embryos. Myonuclei are abnormally rounded (arrows), and there is increased space between fibers (*). (C) Toluidine blue stained semi-thin sections from 72 hpf morphants. Myonuclei from myotubularin morphants are large, abnormally rounded, and contain discrete nucleoli (arrows). Sarcomeric units, however, are normal in appearance. Scale bar = 20 mm.