ZFIN Image: Gribble et al., 2009, Fig. 8

Image

Figure Caption

Fig. 8 Tcf3 binds to regulatory regions of sox4a in vivo. (A) A Tcf3a polyclonal antibody detects a specific band that is absent from tcf3 morphants, and detects larger bands in ΔTcf3-expressing embryo lysates. Multiple bands are most likely to be due to rearranged copies of the transgene. (B) ChIP analysis of whole-embryo lysates shows specific immunoprecipitation of two phylogenetically conserved DNA fragments in regulatory regions of sox4a. A third fragment, lacking nearby Tcf sites, does not precipitate. The conservation plot is adapted from the UCSC Genome Browser. ChIP fragments are indicated by red bars and potential Tcf-binding sites in conserved regions are indicated by green ovals

Acknowledgments

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