Fig. S3

Fig. S3 mrf4^hu2041 mutation generates a likely null allele. (A) The hu2041 mutation in mrf4 and the resultant truncated protein. (B) Ectopic myogenesis in the head is induced by injection of wild-type mrf4 mRNA, but not by mrf4^hu2041 mutant mRNA. The full-length coding sequences of mrf4 and mrf4^hu2041 were inserted into the SacI-SalI sites of pβUT3 (gift from Dr Adam Rodaway, KCL). Capped mRNAs were transcribed from linearised plasmid using the T3 mMESSAGE mMACHINE Kit (Ambion), 100 pg/embryo injected at the 1- to 2-cell stage and embryos analysed by MyHC immunodetection at 24 hpf.