ZFIN ID: ZDB-IMAGE-090220-4
Figures for Ota et al., 2009

Figure Caption/Comments:

Fig. 5 Effects of two types of dominant-negative FGFR3c on the patterning in early gastrulae. Embryos were injected with mRNA (300 pg/embryo) for egfp, mdn-R3, or sdn-R3, and examined for the expression of regional marker genes at 50% epiboly or shield stage. All are animal pole views with dorsal to the right. When the injected embryos showed anomalies compared to control, the numbers of abnormal embryos against those that were stained are shown at the right top. The expression of ntl and tbx6 in the blastoderm margin was downregulated by both dominant-negative genes. gsc expression was repressed only with mdn-R3, and the expression of spt and eve1 in the ventral margin was little affected. Scale bars, 100 μm.

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