Dose-dependent effects of mdn-R3 on the anterior–posterior patterning of the embryo. (A–D) Embryos were injected with egfp mRNA (500 pg/embryo, (A)) or different doses of mdn-R3 mRNA (160–640 pg/embryo) and stained at the bud stage for six3b in the forebrain (asterisk) and pax2a in the MHB (triangle)/otic vesicle (OV)/pronephros. In the views shown here, pax2a expression in the pronephros cannot be seen. (E) Progressive disruption of the embryonic anterior–posterior pattern with increasing doses of mdn-R3 mRNA. Red, only six3b expression in the forebrain was observed; green, the expression of six3b in the forebrain and that of pax2a in the MHB were observed; white, the expression of six3b in the forebrain and that of pax2a in the MHB and OV were observed; blue, the expression of six3b in the forebrain and pax2a in the MHB/OV/pronephros normally occurred. Scale bars, 100 μm.
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